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Image Search Results
Journal: Stem Cells International
Article Title: Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors
doi: 10.1155/2017/1513281
Figure Lengend Snippet: Pluripotent marker expression analysis of hOB-iPSCs and hFB-iPSCs. (a) hOB-iPSC and hFB-iPSC qRT-PCR expression of endogenous pluripotent genes, Sox2, Oct4, Nanog, and hTERT ( ∗ P < 0.01). (b) Immunofluorescence of hOB-iPSC for pluripotency markers, TRA-1-60, Oct4, Nanog, Sox2, and IgG-FITC control (scale bars = 30 μ m). ( ∗ indicates nonstaining MEF background.)
Article Snippet: After isolation from the bone chips, hOBs were maintained in the media for 10–15 days without passaging (passage 0) before qRT-PCR analysis and passaging onto separate culture plates for calcium deposition assays. iPSCs were cultured on
Techniques: Marker, Expressing, Quantitative RT-PCR, Immunofluorescence, Control
Journal: Theranostics
Article Title: High OGT activity is essential for MYC-driven proliferation of prostate cancer cells
doi: 10.7150/thno.30834
Figure Lengend Snippet: Identification of transcription factors that bind to O-GlcNAc marked chromatin. A) Distribution of differently modified histone 3 reads within ±2kb of O-GlcNAc peak. B) . The top 3 motifs enriched for the O-GlcNAc ChIP-seq data showing similarity with known previously identified transcription factor motifs from published datasets. C) O-GlcNAc ChIP-seq consensus overlap with MYC ChIP-seq data reported in this study. D) O-GlcNAc ChIP-seq consensus overlap with MYC ChIP-exo dataset reported previously (GSE73994). E) OSMI-2 disrupts interaction between MYC and HCF-1. Immunoprecipitation (IP) of MYC. IgG denotes negative control. MYC was overexpressed by addition of doxycycline using the LNCaP-MYC cell line for 4 hours either in the presence or absence of 40µM OSMI-2. Experiment was repeated four times. F) Knockout of OGT disrupts the interaction between MYC and HCF-1. Experiment was performed in a mouse embryonic fibroblast cell line that has been genetically engineered to enable removal of OGT gene by addition of 0.5µM Tamoxifen (Tam). After two days of DMSO- or Tam-treatments, cell lysates were prepared and used for immunoprecipitation. Data shown is representative of two biological replicates. G) Overlap of O-GlcNAc (this study), MYC (this study) and HCF-1 (ENCSR000ECH) ChIP-seq data.
Article Snippet:
Techniques: Modification, ChIP-sequencing, Immunoprecipitation, Negative Control, Knock-Out